Journal: Communications Biology

Article Title: PIEZO1 and PECAM1 interact at cell-cell junctions and partner in endothelial force sensing

doi: 10.1038/s42003-023-04706-4

Figure Lengend Snippet: HUVEC data. a – f Cells cultured in a confluent monolayer treated with a control (Ctrl) or b PIEZO1 siRNA and subjected to extracellular Ca 2+ switch assay: pre-treatment (normal Ca 2+ ); −calcium (30 min Ca 2+ -depletion); and recovery (+calcium (post); 30 min after restoring Ca 2+ ). After treatments, cells were stained with anti-CDH5 antibody (αCDH5) (green). The scale bars are 100 µm. c Images are enlarged from the white boxes in a , b +calcium (post). To the right are line-intensity plots (black) with a Gaussian fit (red) used to calculate peak width at half maximum for the superimposed grey lines shown in the enlarged images. d , e As for a and b but showing box plots for +calcium pre-treatment ( n = 3, P = 0.92988) and +calcium (post) ( n = 3, *** P = 8.86242 ×10 −5 ) conditions. Superimposed data points are measurements from individual cell-cell junctions for +calcium (pre) (Ctrl si = 89, PIEZO1 si = 90) and +calcium (post) (Ctrl si = 87, PIEZO1 si = 88). f Enlarged and merged images of CDH5 (green, from a , b ) and F-actin (phalloidin) (magenta, from Supplementary Fig. ) staining for the calcium switch recovery (+calcium (post); 30 min after restoring Ca 2+ ) conditions in Ctrl or PIEZO1 siRNA treated HUVECs. Scale bars are 25 µm g Schematic representation of F-actin and CDH5 with (+) and without (−) PIEZO1 or PIEZO1 activation by mechanical force, based on the data of a – f and Supplementary Fig. . In the +PIEZO1 condition, there is suggested to be junctional remodelling with more radial actin and wider (less tight and more leaky) junctions.

Article Snippet: Images were prepared for particle analysis by using the Gaussian Blur filter (Sigma radius = 0.015 µm in scaled units).

Techniques: Cell Culture, Staining, Activation Assay

Journal: bioRxiv

Article Title: Two disparity channels in human visual cortex with different contrast and blur sensitivity

doi: 10.1101/2023.09.14.557625

Figure Lengend Snippet: Example half-images for contrast variation (top left) and blur variation (bottom left) with corresponding Fourier power spectra. Contrast in the example images is specified on the Michelson definition. The blur levels are specified in terms of the standard deviation of the gaussian kernel in terms of visual angle (arc min). Contrast reduction affects all spatial frequencies while blur manipulation preferentially affects high spatial frequencies.

Article Snippet: Half-image contrast was defined on the Michelson definition in the space domain and blur was generated by convolving the half-images with a Gaussian blur kernel using the MATLAB ‘imgaussfilt’ function.

Techniques: Standard Deviation